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fastquant rt kit with gdnase  (tiangen biotech co)


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    Structured Review

    tiangen biotech co fastquant rt kit with gdnase
    Fastquant Rt Kit With Gdnase, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 96/100, based on 2861 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fastquant+rt+kit+with+gdnase/FastQuant+RT+Kit/pm42883216-287-13-18
    Average 96 stars, based on 2861 article reviews
    fastquant rt kit with gdnase - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Synthesized:

    Article Title: Genome-wide analysis of the aldo-keto reductase (AKR) gene family and the role of PpAKR1 in salicylic acid-mediated delaying sand pear (Pyrus pyrifolia) fruit senescence
    Article Snippet: Postharvest senescence limits the storability and market value of sand pear, yet the role of aldo-keto reductases (AKRs) in this process remains unclear.. Here, we conducted a genome‐wide analysis of the PpAKR superfamily and functionally characterized PpAKR1, previously identified as salicylic acid (SA) responsive in ‘Whangkeumbae’ fruit.. A total of 50 PpAKR genes were identified and categorized into four families-AKR2, AKR4, AKR6, and AKR13-based on phylogenetic analysis.

    Article Title: Ethylene modulates cell wall mechanics for root responses to compaction
    Article Snippet: Total RNA was extracted using Trizol reagent (Invitrogen) followed by purification with chloroform/isopentyl alcohol. .. The cDNA was synthesized using the FastQuant RT Kit with gDNase (Tiangen Biotech). .. RT–qPCR was conducted using the SYBR Green Premix Pro Taq HS qPCR Kit (Accurate Biotechnology).

    Article Title: Ethylene modulates cell wall mechanics for root responses to compaction.
    Article Snippet: Total RNA was extracted using Trizol reagent (Invitrogen) followed by purification with chloroform/isopentyl alcohol. .. The cDNA was synthesized using the FastQuant RT Kit with gDNase (Tiangen Biotech). .. RT–qPCR was conducted using the SYBR Green Premix Pro Taq HS qPCR Kit (Accurate Biotechnology).

    Quantitative RT-PCR:

    Article Title: Identification of transcription factors regulating starch biosynthesis in maize through integrated GWAS and transcriptomic analysis.
    Article Snippet: RNA integrity was assessed using 1.5% agarose gel electrophoresis and NanoDrop spectrophotometry (A260/A280 > 1.8). .. First-strand cDNA was synthesised from 1 μg total RNA using the FastQuant RT Kit with gDNase (Tiangen). qRT-PCR was performed on a 7500 Real-Time PCR System (Applied Biosystems) using three biological replicates and three technical replicates per sample. .. Gene-specific primers (Table S10) were designed using Primer 5.0 (https://www.premierbiosoft.com/primerdesign/).

    Article Title: NR5A nuclear receptors coordinately regulate locust metamorphic transition and fecundity.
    Article Snippet: NR5A nuclear receptors Ftz-f1 and HR39 are evolutionarily conserved with essential roles in insect development and reproduction, yet their divergent and isoform-specific functions in metamorphosis and reproduction remain unclear.. Besides a single HR39 transcript, two Ftz-f1 transcript variants are identified in diverse insect species including Locusta migratoria.. The locust paralogues exhibit spatiotemporal fluctuations in mRNA levels, with LmαFtz-f1 and LmHR39 transcripts being more abundant than LmβFtz-f1, and at higher levels in adults than nymphs.

    Real-time Polymerase Chain Reaction:

    Article Title: Identification of transcription factors regulating starch biosynthesis in maize through integrated GWAS and transcriptomic analysis.
    Article Snippet: RNA integrity was assessed using 1.5% agarose gel electrophoresis and NanoDrop spectrophotometry (A260/A280 > 1.8). .. First-strand cDNA was synthesised from 1 μg total RNA using the FastQuant RT Kit with gDNase (Tiangen). qRT-PCR was performed on a 7500 Real-Time PCR System (Applied Biosystems) using three biological replicates and three technical replicates per sample. .. Gene-specific primers (Table S10) were designed using Primer 5.0 (https://www.premierbiosoft.com/primerdesign/).

    Article Title: Reduction of Buchnera with rifampicin impairs the density-dependent induction of winged morph in pea aphid.
    Article Snippet: Wing dimorphism is a critical trait that helps insects better adapt to environments.. High population density and poor nutrition are known to induce winged morph in aphids.. Buchnera aphidicola, an obligate endosymbiont of aphids, supplies the host with essential amino acids and B vitamins, while the mechanistic basis of Buchnera’s role in density-dependent production of winged aphids is largely unclear.

    Concentration Assay:

    Article Title: Identification of a novel and high-affinity cyclic peptide targeting Keap1 for inflammation treatment by a combined virtual screening strategy
    Article Snippet: The TRIzol reagent (Invitrogen, Carlsbad, CA, USA) was added to lyse the cells. .. The concentration and purity of total RNA were measured using a spectrophotometer. cDNA was synthesised using the FastQuant RT Kit with gDNase (Tiangen Biotech Co., Ltd., Beijing, China) strictly according to the manufacturer’s instructions. ..

    Spectrophotometry:

    Article Title: Identification of a novel and high-affinity cyclic peptide targeting Keap1 for inflammation treatment by a combined virtual screening strategy
    Article Snippet: The TRIzol reagent (Invitrogen, Carlsbad, CA, USA) was added to lyse the cells. .. The concentration and purity of total RNA were measured using a spectrophotometer. cDNA was synthesised using the FastQuant RT Kit with gDNase (Tiangen Biotech Co., Ltd., Beijing, China) strictly according to the manufacturer’s instructions. ..

    cDNA Synthesis:

    Article Title: Reduction of Buchnera with rifampicin impairs the density-dependent induction of winged morph in pea aphid.
    Article Snippet: Wing dimorphism is a critical trait that helps insects better adapt to environments.. High population density and poor nutrition are known to induce winged morph in aphids.. Buchnera aphidicola, an obligate endosymbiont of aphids, supplies the host with essential amino acids and B vitamins, while the mechanistic basis of Buchnera’s role in density-dependent production of winged aphids is largely unclear.

    Reverse Transcription:

    Article Title: NR5A nuclear receptors coordinately regulate locust metamorphic transition and fecundity.
    Article Snippet: NR5A nuclear receptors Ftz-f1 and HR39 are evolutionarily conserved with essential roles in insect development and reproduction, yet their divergent and isoform-specific functions in metamorphosis and reproduction remain unclear.. Besides a single HR39 transcript, two Ftz-f1 transcript variants are identified in diverse insect species including Locusta migratoria.. The locust paralogues exhibit spatiotemporal fluctuations in mRNA levels, with LmαFtz-f1 and LmHR39 transcripts being more abundant than LmβFtz-f1, and at higher levels in adults than nymphs.

    SYBR Green Assay:

    Article Title: NR5A nuclear receptors coordinately regulate locust metamorphic transition and fecundity.
    Article Snippet: NR5A nuclear receptors Ftz-f1 and HR39 are evolutionarily conserved with essential roles in insect development and reproduction, yet their divergent and isoform-specific functions in metamorphosis and reproduction remain unclear.. Besides a single HR39 transcript, two Ftz-f1 transcript variants are identified in diverse insect species including Locusta migratoria.. The locust paralogues exhibit spatiotemporal fluctuations in mRNA levels, with LmαFtz-f1 and LmHR39 transcripts being more abundant than LmβFtz-f1, and at higher levels in adults than nymphs.

    Amplification:

    Article Title: NR5A nuclear receptors coordinately regulate locust metamorphic transition and fecundity.
    Article Snippet: NR5A nuclear receptors Ftz-f1 and HR39 are evolutionarily conserved with essential roles in insect development and reproduction, yet their divergent and isoform-specific functions in metamorphosis and reproduction remain unclear.. Besides a single HR39 transcript, two Ftz-f1 transcript variants are identified in diverse insect species including Locusta migratoria.. The locust paralogues exhibit spatiotemporal fluctuations in mRNA levels, with LmαFtz-f1 and LmHR39 transcripts being more abundant than LmβFtz-f1, and at higher levels in adults than nymphs.



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